qiagen EpiTect Fast DNA Bisulfite Kit快速亚硫酸氢盐转化试剂盒
qiagen EpiTect Fast DNA Bisulfite Kit快速亚硫酸氢盐转化试剂盒
EpiTect Fast Bisulfite Conversion Kits可在少至30分钟内将未甲基化的胞嘧啶高效转化成尿嘧啶，起始材料选择也有出色的灵活性。简化的样本裂解操作流程和直接的DNA亚硫酸氢盐转化使转化过程快速、省力，样本包括全血、培养细胞、组织样本、基因组DNA和福尔马林固定石蜡包埋（FFPE）切片。创新的DNA Protect技术确保有效的DNA变性而不会降解，得到高产量DNA和可重复性结果。此外，转化DNA可在少至10 µl洗脱缓冲液中洗脱，得到高浓度DNA。
qiagen EpiTect Fast DNA Bisulfite Kit快速亚硫酸氢盐转化试剂盒
Performance
高效亚硫酸氢盐转化
在甲基化分析中，对DNA亚硫酸氢盐处理，将未甲基化的胞嘧啶转化成胞嘧啶是zui关键的步骤，因为反应效率会对下游分析的可靠性造成很大影响。EpiTect Fast Bisulfite Conversion Kits含有的创新型Bisulfite Solution可进行超快速、高效的亚硫酸氢盐转化，甚至可处理难分析的样本，如来自女性样本的X染色体基因组DNA，因为2个X染色体中有一个是高度甲基化的（参见"Highly efficient cytosine conversion of X chromosome DNA"）。焦磷酸测序对胞嘧啶转化定量分析表明，与其他供应商的试剂盒相比，EpiTect Fast Bisulfite Conversion Kit的转化效果明显更好，特别是当起始材料的量很有*（参见"Superior bisulfite conversion"）。
订购信息：
Cat No./ID: 59802 EpiTect Fast Bisulfite Kit (10) Log in Trial kit for 10 preps: Deparaffinization Solution, Lysis Buffer, Proteinase K, Bisulfite Solution, DNA Protect Buffer, MinElute DNA Spin Columns, Carrier RNA, and Buffers |
Cat No./ID: 59824 EpiTect Fast DNA Bisulfite Kit (50) Log in For 50 preps: Bisulfite Solution, DNA Protect Buffer, MinElute DNA Spin Columns, Carrier RNA, and Buffers |
Cat No./ID: 59826 EpiTect Fast DNA Bisulfite Kit (200) Log in For 200 preps: Bisulfite Solution, DNA Protect Buffer, MinElute DNA Spin Columns, Carrier RNA, and Buffers |
Cat No./ID: 59864 EpiTect Fast LyseAll Bisulfite Kit (50) Log in For 50 preps: Lysis Buffer, Proteinase K, Bisulfite Solution, DNA Protect Buffer, MinElute DNA Spin Columns, Carrier RNA, and Buffers |
Cat No./ID: 59866 EpiTect Fast LyseAll Bisulfite Kit (200) Log in For 200 preps: Lysis Buffer, Proteinase K, Bisulfite Solution, DNA Protect Buffer, MinElute DNA Spin Columns, Carrier RNA, and Buffers |
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